Hi,
I am trying to run velocyto using run10x command. I'm running on cluster by requesting 60 GB memory. Do I need more memory for that? Thank you so much for your help.

initial position.
2019-04-29 20:09:26,261 - DEBUG - 2745314 reads were skipped because no apropiate cell or umi barcode was found
2019-04-29 20:09:26,262 - INFO - Now just waiting that the bam sorting process terminates
Traceback (most recent call last):
File "/home/mrr2006/.conda/envs/velocyto/bin/velocyto", line 11, in
sys.exit(cli())
File "/home/mrr2006/.conda/envs/velocyto/lib/python3.6/site-packages/click/core.py", line 722, in call
return self.main(*args, **kwargs)
File "/home/mrr2006/.conda/envs/velocyto/lib/python3.6/site-packages/click/core.py", line 697, in main
rv = self.invoke(ctx)
File "/home/mrr2006/.conda/envs/velocyto/lib/python3.6/site-packages/click/core.py", line 1066, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File "/home/mrr2006/.conda/envs/velocyto/lib/python3.6/site-packages/click/core.py", line 895, in invoke
return ctx.invoke(self.callback, **ctx.params)
File "/home/mrr2006/.conda/envs/velocyto/lib/python3.6/site-packages/click/core.py", line 535, in invoke
return callback(*args, **kwargs)
File "/home/mrr2006/.conda/envs/velocyto/lib/python3.6/site-packages/velocyto/commands/run10x.py", line 115, in run10x
samtools_memory=samtools_memory, dump=dump, loom_numeric_dtype=dtype, verbose=verbose, additional_ca=additional_ca)
File "/home/mrr2006/.conda/envs/velocyto/lib/python3.6/site-packages/velocyto/commands/_run.py", line 225, in _run
Otherwise sort manually by samtools sort -l [compression] -m [mb_to_use]M -t [tagname] -O BAM -@ [threads_to_use] -o cellsorted_[bamfile] [bamfile]")
MemoryError: bam file #0 could not be sorted by cells.
This is probably related to an old version of samtools, please install samtools >= 1.6. In alternative this could be a memory error, try to set the --samtools_memory option to a value compatible with your system. Otherwise sort manually by samtools sort -l [compression] -m [mb_to_use]M -t [tagname] -O BAM -@ [threads_to_use] -o cellsorted_[bamfile] [bamfile]