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q2-assembly

CI codecov Code style: black

QIIME 2 plugin for (meta)genome assembly.

Installation

q2-assembly is available as part of the QIIME 2 moshpit distribution. For installation and usage instructions please consult the official QIIME 2 documentation.

Functionality

This QIIME 2 plugin contains actions used to assemble (meta)genomes from short single/paired-end sequencing reads:

Action Description Underlying tool
assemble-megahit Assemble contigs using MEGAHIT. MEGAHIT
assemble-spades Assemble contigs using SPAdes. SPAdes
evaluate-contigs Evaluate quality of the assembled contigs using metaQUAST. QUAST
generate-reads Simulate NGS reads using InSilicoSeq. InSilicoSeq
index-contigs Index contigs using Bowtie 2. Bowtie 2
index-derep-mags Index dereplicated MAGs using Bowtie2. Bowtie 2
index-mags Index MAGs using Bowtie 2. Bowtie 2
map-reads Map reads to contigs/MAGs using Bowtie 2. Bowtie 2
rename-contigs Rename contigs using unique IDs. -

Dev environment

This repository follows the black code style. To make the development slightly easier there are a couple of pre-commit hooks included here that will ensure that your changes follow that formatting style. Before you start working on the code, please install the hooks by executing make dev in your conda environment. From then on, they will be run automatically every time you commit any changes.