Remove the polyA bases of Single cell RNA sequencing data generated by Fluidigm’s C1 mRNA Seq HT IFC platform.
platform: python2,or add '()' for print
inputfile: raw fastq data from NGS plateform
outputfile: a fastqfile with removed polyA and a record file
The final result can be checked with fastp software :http://opengene.org/fastp/fastp.html
Please attention that this script need mathematical theory to support.
Whether you have question or suggestions, please let me know: yy1036832160@163.com
The following analysis could be conducted as in papers: align by STAR, quantified by featureCounts, defference analysis by Monocle, GO/KEGG analisys by clusterProfiler。